ALK

This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain.  This gene has been found to be rearranged, mutated, or amplified in a series of tumors including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).

Reference: www.ncbi.nlm.nih.gov/gene/238

Assay type: Deep Sequencing
What is analyzed: Mutational analysis of exon 19-28
Platform: MiSeq (Illumina)
Tissue Type: Lung carcinoma
Required material: FFPE tissue; DNA

Assay type: Fluorescence in situ hybridization (FISH)
What is analyzed: ALK Translocation
Platform: Thermobrite (Abbott Molecular)
Kit/probe: Abott/ Vysis ALK break Apart
Tissue Type: Lung carcinoma
Required material: FFPE tissue