E-Cadherin

E-Cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites.

Reference: http://www.ncbi.nlm.nih.gov/gene/999

Assay type: Immunohistochemistry (IHC)
What is analyzed: Semi-quantitative detection of  human E-cadherin protein
Platform: VENTANA BenchMark® XT
Kit/antibody: Clon ECH-6, Zytomed Systems Berlin, #MSK033
Tissue Type: Various solid tumours
Required material: FFPE tissue