EGFR

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.

Referencehttp://www.ncbi.nlm.nih.gov/gene/1956

Assay type: Immunohistochemistry (IHC)
What is analyzed: Semi-quantitative detection of epidermal growth factor receptor
Platform
: Dako Autostainer  Plus & Autostainer LinkKit/antibody: EGFR pharmDX Kit (Dako)
Tissue Type: Breast, Lung, Gastric  and Colorectal carcinoma
Required material: FFPE tissue

Assay type: Mutation Analyses
What is analyzed: Epidermal growth factor receptor common mutations (exons 18-21)
Platform: Rotorgene
Kit/antibody: therascreen® EGFR RGQ PCR Kit Test (Qiagen)
Tissue Type: Lung carcinoma
Required material: FFPE tissue

Assay type: Fluorescence in situ hybridization (FISH)
What is analyzed
: Epidermal growth factor receptor amplifications
Platform
: Hybridizer (Dako), Thermobrite (Vysis)
Kit/probe
: Vysis EGFR/CEP7 FISH Probe kit (Abbott Molecular)
Tissue Type
: Lung carcinoma
Required material
: FFPE tissue

Assay type: Deep/Sanger Sequencing
What is analyzed
: Mutational analysis of exons 18-21
Platform
: MiSeq (Illumina), Genetic Analyzer 3500 (ABI)
Kit/antibody
: Terminator v1.1 Cycle Sequencing Kit (ABI)
Tissue Type
: Lung carcinoma
Required material
: FFPE tissue; DNA