KRAS

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

Reference: http://www.ncbi.nlm.nih.gov/gene/3845

Assay type: Mutation Analysis
What is analyzed: Common gene Mutations in KRAS codon 12 and 13
Platform: Rotor-Gene Q Instrument
Kit/antibody: therascreen® K-Ras RGQ PCR Kit (Qiagen)
Tissue Type: Lung, Colon carcinoma
Required material: FFPE tissue; DNA

Assay type: Deep/Sanger Sequencing; High resolution melting
What is analyzed: Mutational analysis of exon 2 and 3
Platform: MiSeq (Illumina), LightCycler 480 (Roche), Genetic Analyzer 3500 (ABI)
Kit/antibody: High Resolution Melting Master (Roche); Big Dye Terminator v1.1 Cycle Sequencing Kit (ABI)
Tissue Type: Lung, Colon carcinoma
Required material: FFPE tissue; DNA