Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Anti-Histone H3 (phospho S10) antibody detects Histone H3 phosphorylated on Serine 10 only.


Assay type: Immunohistochemistry (IHC)
What is analyzed: Semi-quantitative detection of  human phosphorylated Histone H3 protein
Platform: VENTANA BenchMark® XT
Kit/antibody: Clone E173, Abcam®, #ab32107
Tissue Type: Various solid tumours
Required material: FFPE tissue