PIK3CA

Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.

Reference: http://www.ncbi.nlm.nih.gov/gene/5290

Assay type: Deep/Sanger sequencing, High resolution melting
What is analyzed: Mutational analysis of exons 2, 9, 20
Platform: MiSeq (Illumina), LightCycler 480 (Roche), Genetic Analyzer 3500 (ABI)
Kit/antibody: High Resolution Melting Master (Roche); Big Dye Terminator v1.1 Cycle Sequencing Kit (ABI)
Tissue Type: Lung carcinoma
Required material: FFPE tissue; DNA