This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway.


Assay type: Immunohistochemistry (IHC)
What is analyzed: Semi-quantitative detection of Phosphatase and tensin homolog protein (PTEN)
Platform: VENTANA BenchMark® XT
Kit/antibody: Clone 138G6 (Cell Signaling Technologies┬«)
Tissue Type: Breast, Gastric, Ovary, Colorectal carcinoma
Required material: FFPE tissue

Assay type: Deep sequencing
What is analyzed: Mutational analysis of exon 7
Platform: MiSeq (Illumina)
Tissue Type: Lung carcinoma
Required material: FFPE tissue; DNA