VEGFR2

Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated. Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2. Phosphorylation at Tyr1212 provides a docking site for GRB2 binding and phospho-Tyr1175 binds the p85 subunit of PI3 kinase and PLC╬│, as well as Shb. Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo.

Reference: http://www.cellsignal.com/products/2479.html

Assay type: Immunohistochemistry (IHC)
What is analyzed: Semi-quantitative detection of human VEGFR2 protein
Platform: VENTANA benchmark® XT
Kit/antibody: Cell Signaling, 2479L)
Tissue Type: Gastric carcinoma, Colorectal carcinoma; NSCLC; Sarcoma; Breast carcinoma
Required material: FFPE